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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

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Author Spotlight: Advancements in iPSCs and Genetic Disease Research

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Minimum criteria for defining induced mesenchymal stem cells.

Mahmood S Choudhery¹, Ruhma Mahmood², David T Harris³

¹Department of Human Genetics & Molecular Biology, University of Health Sciences, Lahore, Pakistan.

Cell Biology International

|March 16, 2022

Summary

Induced mesenchymal stem cells (iMSCs) offer a promising alternative to primary mesenchymal stem cells (MSCs). New criteria are proposed to accurately define iMSCs, ensuring their safe and effective clinical application.

Keywords:

additional characterization clinical applications iMSCs iPSCs minimum criteria primary MSCs

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Last Updated: Sep 30, 2025

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Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model

Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model

Published on: August 10, 2018

Area of Science:

Cellular biology and regenerative medicine

Background:

Mesenchymal stem cells (MSCs) show therapeutic promise but face limitations like low yield and heterogeneity.
Induced mesenchymal stem cells (iMSCs), derived from induced pluripotent stem cells (iPSCs), are emerging as a viable alternative source.

Purpose of the Study:

To address the need for a distinct definition of iMSCs, separate from primary MSCs.
To propose a set of minimum criteria for characterizing iMSCs to ensure uniformity and safety in research and clinical applications.

Main Methods:

Review and analysis of existing MSC characterization criteria (ISCT).
Proposal of additional characterization parameters specific to iMSCs, including morphology, surface marker expression, differentiation potential, teratoma formation, and paracrine factor release.

Main Results:

Current iMSC definitions rely on primary MSC criteria, overlooking their unique nature.
Proposed iMSC criteria include specific markers (CD29, CD44, CD73, CD90, CD105), lack of iPSC factors, trilineage potential, absence of teratoma formation, and secretion of relevant factors.

Conclusions:

Establishing minimum criteria for iMSCs is crucial for consistent identification and progress in the field.
Additional characterization of iMSCs is essential before clinical application to prevent recipient complications.