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Related Experiment Video

Updated: Sep 30, 2025

Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry

Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry

Published on: May 24, 2024

Mitochondrial Membrane Potential Assay.

Srilatha Sakamuru¹, Jinghua Zhao¹, Matias S Attene-Ramos²

¹National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 16, 2022

Summary

This summary is machine-generated.

This study optimized a cell-based assay to measure mitochondrial membrane potential (MMP) in various cell types. This assay effectively screens compounds for mitochondrial toxicity, aiding in cell health assessments.

Keywords:

1536-well plate Mesoxalonitrile 4-trifluoromethoxyphenylhydrazone (FCCP)Mitochondrial membrane potential (MMP)Mitochondrial membrane potential indicator (m-MPI)Mitochondrial toxicity

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Published on: September 18, 2014

Area of Science:

Biochemistry
Cell Biology
Toxicology

Background:

Mitochondrial function is crucial for cell health and is often evaluated by monitoring mitochondrial membrane potential (MMP).
Cationic fluorescent dyes are standard tools for assessing MMP.
Assessing MMP provides insights into cellular metabolic state and potential toxicity.

Purpose of the Study:

To optimize a homogenous cell-based assay for detecting changes in MMP.
To validate the assay's utility in various cell lines (HepG2, HepaRG, AC16).
To establish a high-throughput screening method for identifying compounds with mitochondrial toxicity.

Main Methods:

Utilized a water-soluble mitochondrial membrane potential indicator (m-MPI).
Developed and optimized a homogenous cell-based assay.
Performed the assay in a 1536-well plate format for high-throughput screening.

Main Results:

Successfully detected changes in MMP across different cell types.
The optimized assay demonstrated robustness and reproducibility.
The assay is suitable for screening compound libraries for effects on MMP.

Conclusions:

The developed homogenous cell-based MMP assay is an effective tool for evaluating mitochondrial toxicity.
This assay facilitates the screening of compound libraries to identify potential mitochondrial toxicants.
The method supports the assessment of cell health by monitoring mitochondrial function.