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Glucose and Ethanol Detection with an Affinity-Switchable Lateral Flow Assay.

Hsiao-Jung Huang¹, Yu-Ting Lin¹, Min-Chi Chung¹

¹Department of Chemistry, National Tsing Hua University, 101 Section 2, Kuang Fu Road, Hsinchu 30013, Taiwan.

Analytical Chemistry

|March 17, 2022

Summary

A new affinity-switchable lateral flow assay (ASLFA) enables rapid detection of hydrogen peroxide, glucose, and ethanol. This innovative approach overcomes limitations of traditional assays for small molecules in samples like blood and urine.

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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

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Published on: November 13, 2021

Area of Science:

Analytical Chemistry
Biotechnology
Biosensors

Background:

Lateral flow assays (LFAs) are widely used for rapid, on-site detection but struggle with small molecules.
Traditional LFAs often rely on antibody-antigen interactions, limiting their application for certain analytes.

Purpose of the Study:

To develop an affinity-switchable lateral flow assay (ASLFA) for sensitive detection of hydrogen peroxide, glucose, and ethanol.
To demonstrate the ASLFA's effectiveness in complex biological samples like blood serum and urine.

Main Methods:

Utilized a gold nanoparticle-conjugated affinity-switchable biotin probe (Au@H2O-ASB).
The probe's binding to streptavidin (SA) is activated by hydrogen peroxide (H2O2).
Glucose and ethanol detection is achieved indirectly via H2O2 generation using specific oxidases.

Main Results:

The ASLFA successfully detected hydrogen peroxide, glucose, and ethanol with high selectivity.
The assay demonstrated applicability in complex matrices such as blood serum and urine samples.
The affinity-switchable mechanism provides a novel approach for LFA development.

Conclusions:

The developed ASLFA offers a versatile and rapid alternative for detecting small molecules like glucose and ethanol.
This method addresses a key challenge in LFA technology, expanding its potential applications.
ASLFA presents a promising platform for simple, on-site diagnostic tests.