Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Complementation Tests

Complementation Tests

A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Durum Wheat cv. Svevo Reference Genome Rel.2.0: A Comprehensive Tool for Wheat Genomics.

Plant biotechnology journal·2026

Same author

Gene expression landscapes driving early life stages of the keystone seagrass Posidonia oceanica.

Plant cell reports·2026

Same author

Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance.

The Plant cell·2026

Same author

Molecular mimicry of a pathogen virulence target by a plant immune receptor.

Science (New York, N.Y.)·2026

Same author

Genetic dissection of root traits in barley identifies major QTLs and domestication signature.

Plant cell reports·2026

Same author

On the state of protein function prediction: a report on the fourth CAFA challenge.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: Sep 29, 2025

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Cloning the barley nec3 disease lesion mimic mutant using complementation by sequencing.

Serena Rosignoli¹, Francesco Cosenza^1,2, Matthew J Moscou²

¹Dep. of Agricultural and Food Sciences, Univ. of Bologna, Viale G. Fanin 44, Bologna, Italy, 40127.

The Plant Genome

|March 18, 2022

Summary

Researchers identified a new gene, CYP71P1, responsible for disease lesion mimic (DLM) phenotypes in barley using a novel sequencing method. This discovery aids in understanding plant defense mechanisms and host-pathogen interactions.

More Related Videos

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

Related Experiment Videos

Last Updated: Sep 29, 2025

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

Area of Science:

Plant genetics
Molecular biology
Crop science

Background:

Disease lesion mimic (DLM) mutants exhibit necrosis without pathogen infection, offering insights into plant defense pathways.
Traditional genetic strategies for identifying causal genes are time-consuming and resource-intensive.

Purpose of the Study:

To describe a collection of barley DLM mutants.
To introduce and validate a novel method, complementation by sequencing (CBS), for efficient gene identification in mutagenized populations.
To identify the gene responsible for specific DLM phenotypes in barley.

Main Methods:

Generation and characterization of 34 barley DLM mutants.
Application of complementation by sequencing (CBS) to identify causative genes.
Comparative phylogenetic analysis of identified gene families.

Main Results:

Successfully identified the cytochrome P450 CYP71P1 gene as responsible for orange blotch DLM mutants in barley.
Demonstrated that CBS is a cost-effective approach for cloning genes controlling phenotypes in chemically mutagenized collections.
Phylogenetic analysis revealed early emergence and recurrent loss of the CYP71P1 gene family in angiosperms.

Conclusions:

Complementation by sequencing (CBS) provides a powerful and efficient tool for gene discovery in plant research.
The identified CYP71P1 gene is crucial for understanding barley DLM phenotypes and potentially plant-pathogen interactions.
The TILLMore (TM) collection, combined with CBS, will advance research into DLM mechanisms and plant immunity.