Large field-of-view non-invasive imaging through scattering layers using fluctuating random illumination

Area of Science:

• Biomedical Optics
• Optical Imaging
• Photonics

Background:

• Non-invasive optical imaging is crucial for diagnostics but limited by scattering media.
• Existing techniques struggle with large fields of view beyond the optical memory effect, especially without wavefront shaping.

Purpose of the Study:

• To develop a non-invasive fluorescence imaging method for scattering media.
• To extend the field of view beyond the physical limits of the optical memory effect.
• To provide a simple, robust technique without requiring specialized equipment like spatial light modulators.

Main Methods:

• Utilized demixing of speckle patterns from fluorescent objects under variable, unknown random illumination.
• Employed matrix factorization and a novel fingerprint-based reconstruction algorithm.
• Validated experimentally with diverse fluorescent samples.

Main Results:

• Successfully demonstrated non-invasive fluorescence imaging behind scattering layers.
• Achieved field-of-views extending up to three times the range of the optical memory effect.
• Confirmed the method's efficiency and robustness across various samples.

Conclusions:

• The developed technique enables non-invasive imaging with an extended field of view, surpassing optical memory effect limitations.
• The method is simple, requires no spatial light modulator or guide star, and is adaptable to various imaging schemes.
• This approach offers a significant advancement for optical imaging in scattering environments.