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miR-93-5p suppresses ovarian cancer malignancy and negatively regulate CCND2 by binding to its 3'UTR region

Guotong Chen1, Yiwei Yan1,2, Xiaojv Qiu1,2

  • 1Department of Obstetrics and Gynecology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

Discover Oncology
|March 20, 2022
PubMed

Insights

MicroRNA-93-5p (miR-93-5p) is downregulated in ovarian cancer, suppressing tumor growth and migration by targeting Cyclin-D2 (CCND2). This finding identifies miR-93-5p as a potential biomarker for improved ovarian cancer patient outcomes.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Ovarian cancer is a leading cause of cancer-related death globally, with mechanisms of malignancy poorly understood.
  • MicroRNAs (miRNAs) play critical roles in cancer development and progression.
  • Cyclin-D2 (CCND2) is frequently involved in uncontrolled cell proliferation in cancers.

Purpose of the Study:

  • To investigate the role of miR-93-5p in ovarian cancer.
  • To determine if miR-93-5p targets CCND2.
  • To evaluate miR-93-5p as a prognostic biomarker in ovarian cancer.

Main Methods:

  • Bioinformatic analysis to predict miRNA-target interactions.
  • Ovarian cancer patient sample analysis for miRNA and mRNA expression.
  • Luciferase reporter assays to confirm direct binding.
  • Cell proliferation, migration, and apoptosis assays (CCK8, wound healing, flow cytometry).

Main Results:

  • CCND2 was identified as a direct target of miR-93-5p, with inverse correlation in patient samples.
  • miR-93-5p expression was significantly downregulated in ovarian cancer tissues.
  • Downregulation of miR-93-5p correlated with poorer patient survival.
  • miR-93-5p suppressed ovarian cancer cell proliferation and migration while promoting apoptosis.

Conclusions:

  • miR-93-5p acts as a tumor suppressor in ovarian cancer by targeting CCND2.
  • miR-93-5p is a potential diagnostic and prognostic biomarker for ovarian cancer.
  • Targeting miR-93-5p may offer a therapeutic strategy for ovarian cancer.

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