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Related Experiment Video

Updated: Sep 29, 2025

Isolation and Identification of Extravascular Immune Cells of the Heart

Isolation and Identification of Extravascular Immune Cells of the Heart

Published on: August 23, 2018

Pyroptosis Patterns Are Involved in Immune Microenvironment Regulation of Dilated Cardiomyopathy.

Kexin Wang¹, Zhan Lv¹, Chenggang Fang¹

¹Department of Internal Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China.

Disease Markers

|March 21, 2022

Summary

Pyroptosis, a cell death process, significantly impacts the immune environment in dilated cardiomyopathy (DCM). This study identifies key pyroptosis genes and patterns linked to immune responses in DCM, offering new insights into the disease.

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Area of Science:

Cardiovascular Research
Immunology
Cell Death Mechanisms

Background:

Cell pyroptosis plays a crucial role in regulating immunity, particularly in cardiovascular diseases.
The involvement of pyroptosis signaling pathways in the immune microenvironment of dilated cardiomyopathy (DCM) remains largely unexplored.

Purpose of the Study:

To investigate the influence of pyroptosis on the immune microenvironment in dilated cardiomyopathy.
To identify pyroptosis-related genes and patterns associated with immune dysregulation in DCM.

Main Methods:

Analysis of pyroptosis-related gene (PRG) expression in DCM and healthy samples.
Development of a classifier based on hub PRGs to distinguish between DCM and healthy individuals.
RT-PCR validation of differentially expressed hub PRGs.
Correlation analysis between pyroptosis markers and immune cell infiltration/responses.
Identification and characterization of distinct pyroptosis-mediated immune patterns in DCM.

Main Results:

Altered expression of 19 PRGs was observed in DCM samples compared to healthy controls.
A classifier based on 12 hub PRGs effectively distinguished between DCM and healthy samples.
Five hub PRGs showed significant differential expression in DCM.
Specific pyroptosis markers correlated with immune characteristics: GSDMD with pDCs and Tregs, CASP1 with parainflammation, and CASP9 with type II IFN response.
Two distinct pyroptosis-mediated patterns (A and B) were identified, with pattern B associated with a more active immune response and enriched pathways like NOTCH signaling.

Conclusions:

Pyroptosis significantly influences the immune microenvironment in dilated cardiomyopathy.
Identified pyroptosis-related genes and patterns provide novel insights into DCM pathogenesis.
This study highlights pyroptosis as a potential therapeutic target for managing DCM-associated immune dysregulation.