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Downregulation of miR-211-5p Promotes Carboplatin Resistance in Human Retinoblastoma Y79 Cells by Affecting the

Ning Ke1, Lin Chen1, Qing Liu1

  • 1Department of Ophthalmology, Children's Hospital of Chongqing Medical University, Chongqing, China.

Frontiers in Oncology
|March 21, 2022
PubMed
Abstract

Insights

Downregulation of miR-211-5p promotes carboplatin resistance in retinoblastoma cells by affecting the GDNF-LIF interaction. This finding offers potential strategies for reversing drug resistance in retinoblastoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Retinoblastoma is a pediatric eye cancer.
  • Carboplatin is a common chemotherapy agent used for retinoblastoma.
  • Acquired drug resistance limits treatment efficacy.

Purpose of the Study:

  • Investigate the role of the miR-211-5p-GDNF signaling pathway in carboplatin resistance.
  • Identify factors influencing this pathway in retinoblastoma Y79 cells.

Main Methods:

  • Established a carboplatin-resistant retinoblastoma cell line (Y79R).
  • Utilized RNA-seq, microRNA-seq, RT-PCR, Western blot, and flow cytometry.
  • Employed TargetScan, STING, and Co-Immunoprecipitation (COIP) for analysis.

Main Results:

  • GDNF (Glial cell line-derived neurotrophic factor) was identified as a key gene in drug resistance.
  • Downregulation of miR-211-5p increased carboplatin resistance in Y79 cells.
  • GDNF was confirmed as a target of miR-211-5p and interacts with LIF (Leukemia Inhibitory Factor).

Conclusions:

  • Downregulation of miR-211-5p promotes carboplatin resistance in retinoblastoma Y79 cells.
  • The GDNF-LIF interaction influences this drug resistance mechanism.
  • Findings may guide strategies for reversing carboplatin resistance in retinoblastoma.
Keywords:
carboplatin resistanceglial cell-derived neurotrophic factor (GDNF)leukemia inhibitory factor (ILF)miR-211-5pretinoblastoma

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