Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Subviral Agents01:29

Subviral Agents

154
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
154
Amyloid Fibrils03:03

Amyloid Fibrils

10.2K
Amyloid fibrils are aggregates of misfolded proteins.  Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils. 
Amyloid deposits were observed as early as 1639 in the liver and the spleen.   In 1854, Rudolph Virchow performed iodine staining,...
10.2K
Eukaryotic Compartmentalization01:46

Eukaryotic Compartmentalization

164.8K
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
164.8K
Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

18.6K
The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
18.6K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

8.7K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.7K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Science Journals.

Science (New York, N.Y.)·2026
Same author

Self-sustained heterogeneity drives PDAC.

Science signaling·2026
Same author

In Science Journals.

Science (New York, N.Y.)·2026
Same author

In Science Journals.

Science (New York, N.Y.)·2026
Same author

An inflammation memento.

Science signaling·2026
Same author

Fungi facilitate gut healing.

Science signaling·2026
Same journal

ZNRF3 and RNF43 are active monomeric E3 ubiquitin ligases that self-associate.

Science signaling·2026
Same journal

Allosteric ligands with distinct properties uncover tissue-specific physiological regulation mediated by free fatty acid receptor 2.

Science signaling·2026
Same journal

Diacylglycerol kinase ζ in B lymphocytes supports CD40-mediated immune synapse formation, mTORC1 signaling, and plasma cell fate.

Science signaling·2026
Same journal

The APC/C adaptor Cdh1 stabilizes STING to potentiate innate immune activation in renal cell carcinoma.

Science signaling·2026
Same journal

Fattening mother's milk with oxytocin.

Science signaling·2026
Same journal

Virion display reveals MD-1 as an endogenous agonist for the orphan receptor GPRC5B.

Science signaling·2026
See all related articles

Related Experiment Video

Updated: Sep 29, 2025

Protein Misfolding Cyclic Amplification of Prions
10:12

Protein Misfolding Cyclic Amplification of Prions

Published on: November 7, 2012

19.7K

Compartmentalized prions.

Annalisa M VanHook1

  • 1Science Signaling, AAAS, Washington, DC 20005, USA.

Science Signaling
|March 22, 2022
PubMed
Summary
This summary is machine-generated.

The yeast prion-like protein Whi3 is confined to specific endoplasmic reticulum (ER) regions. This localization prevents its inheritance by daughter cells, impacting prion transmission.

More Related Videos

Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
11:29

Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain

Published on: October 3, 2012

10.8K
High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

6.4K

Related Experiment Videos

Last Updated: Sep 29, 2025

Protein Misfolding Cyclic Amplification of Prions
10:12

Protein Misfolding Cyclic Amplification of Prions

Published on: November 7, 2012

19.7K
Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
11:29

Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain

Published on: October 3, 2012

10.8K
High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

6.4K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Biochemistry

Background:

  • Prion-like proteins, such as Whi3 in yeast, can propagate through cells.
  • Understanding the mechanisms that regulate prion-like protein distribution is crucial for cellular inheritance.
  • The endoplasmic reticulum (ER) is a key organelle involved in protein synthesis and trafficking.

Purpose of the Study:

  • To investigate the spatial regulation of the yeast prion-like protein Whi3.
  • To determine how Whi3 localization affects its transmission to daughter cells.

Main Methods:

  • Fluorescence microscopy to visualize Whi3 localization within yeast cells.
  • Genetic manipulation to alter Whi3 distribution.
  • Assays to assess Whi3 transmission to daughter cells.

Main Results:

  • Whi3 was found to be restricted to specific domains within the ER.
  • This restricted localization correlated with a lack of Whi3 transmission to daughter cells.
  • Disrupting Whi3 localization led to its propagation to daughter cells.

Conclusions:

  • Spatial confinement of Whi3 within the ER is a critical mechanism for controlling its inheritance.
  • The ER serves as a barrier to prevent the spread of prion-like proteins.
  • Targeting protein localization offers a potential strategy to manage prion-like protein propagation.