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Natural and Synthetic Sortase A Substrates Are Processed by Staphylococcus aureus via Different Pathways.

Silvie Hansenová Maňásková1,2, Kamran Nazmi1, Wim Van't Hof1

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Staphylococcus aureus sortase A (SrtA) processes native and synthetic substrates differently. Bacitracin, a lipid II inhibitor, decreased native substrate incorporation but increased synthetic substrate incorporation, suggesting distinct pathways for SrtA processing.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Molecular Biology

Background:

  • * *Staphylococcus aureus* sortase A (SrtA) is crucial for anchoring cell wall proteins.
  • * SrtA utilizes a lipid II-dependent pathway for protein incorporation into peptidoglycan.
  • * Previous studies showed SrtA processes exogenous synthetic substrates with varying efficiencies.

Purpose of the Study:

  • * To determine if native and synthetic SrtA substrates are processed via the same pathway.
  • * To investigate the role of lipid II in the processing of different SrtA substrates.

Main Methods:

  • * Assessed the effect of bacitracin, a lipid II inhibitor, on SrtA substrate incorporation.
  • * Compared the incorporation of native protein A and synthetic SrtA substrates in *S. aureus*.

Main Results:

  • * Bacitracin treatment decreased the cell wall incorporation of native protein A.
  • * Bacitracin treatment increased the cell wall incorporation of exogenous synthetic SrtA substrates.
  • * Differential effects of bacitracin suggest distinct processing pathways.

Conclusions:

  • * Native and exogenous synthetic SrtA substrates are processed by *S. aureus* SrtA through different mechanisms.
  • * The lipid II pathway is differentially involved in the processing of native versus synthetic substrates.