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Updated: Sep 29, 2025

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
Published on: December 9, 2020
Examination of Galectin-3 Recruitment into Multivesicular Bodies for Exosomal Secretion
Sebastian Bänfer1, Sophie Kutscher2, Ralf Jacob3
1Department of Cell Biology and Cell Pathology, Philipps University of Marburg, Marburg, Germany. baenfers@staff.uni-marburg.de.
Abstract:
Cells use unconventional secretion to deliver the β-galactoside binding lectin galectin-3 from the cell interior into the extracellular milieu. This process starts with galectin-3 recruitment into intraluminal vesicles (ILVs), which are later released at the plasma membrane as exosomes. Electron microscopy is utilized to determine the location of GFP-tagged galectin-3 in pelleted exosomes. We also describe how these vesicles are harvested from cell culture media to determine their composition. The fluorescent protein GFP was fused with the exosomal sorting motif of galectin-3 to direct GFP into exosomes. Recruitment of this fusion construct into the lumen of exosomes can be assessed by proteinase K accessibility analysis.
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