Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic dissection of the role of <i>Piga</i> and <i>Pgap2</i> in the embryonic mouse brain.

iScience·2026
Same author

The 1 -Cys peroxiredoxin, PRDX-6, suppresses an NHR-49-dependent pro-survival response, including the Flavin monooxygenase, FMO-2, that protects against fungal and bacterial infection.

Redox biology·2026
Same author

Recessive AARS1 variants perturb human and mouse development.

HGG advances·2026
Same author

Gene replacement therapy for Piga GPI-anchor deficiency in the developing nervous system.

bioRxiv : the preprint server for biology·2025
Same author

Mouse variants in Taf1c result in reduced survival to birth.

Developmental biology·2025
Same author

Lipid it up: Freed fats drive ferroptosis.

Cell chemical biology·2025
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Sep 29, 2025

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.2K

Lipid Extraction and Analysis.

Henry H Harrison1, Jennifer L Watts2

  • 1School of Molecular Biosciences, Washington State University, Pullman, WA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 23, 2022
PubMed
Summary
This summary is machine-generated.

We optimized lipid extraction and analysis from Caenorhabditis elegans. This method details lipid class separation and fatty acid profiling in nematodes.

Keywords:
Fatty acid methyl estersGas chromatographyLipid extractionNeutral lipidsPhospholipidsThin-layer chromatography

More Related Videos

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.3K
Determination of Total Lipid and Lipid Classes in Marine Samples
14:59

Determination of Total Lipid and Lipid Classes in Marine Samples

Published on: December 11, 2021

4.8K

Related Experiment Videos

Last Updated: Sep 29, 2025

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.2K
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.3K
Determination of Total Lipid and Lipid Classes in Marine Samples
14:59

Determination of Total Lipid and Lipid Classes in Marine Samples

Published on: December 11, 2021

4.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nematology

Background:

  • Lipids are essential cellular components, serving structural, energetic, and signaling roles.
  • Understanding lipid profiles is crucial for biological research.
  • Caenorhabditis elegans is a key model organism for biological studies.

Purpose of the Study:

  • To establish optimized protocols for lipid extraction and analysis in Caenorhabditis elegans.
  • To enable detailed characterization of lipid composition in different life stages of C. elegans.

Main Methods:

  • Optimized total lipid extraction from C. elegans larvae, adults, and embryos.
  • Thin-layer chromatography (TLC) for separating major lipid classes.
  • Gas chromatography (GC) for analyzing fatty acid composition.

Main Results:

  • Successfully extracted and analyzed total lipids from various C. elegans life stages.
  • Demonstrated effective separation of lipid classes using TLC.
  • Quantified fatty acid profiles from lipid extracts and directly from nematodes.

Conclusions:

  • The described methods provide a robust framework for lipidomic studies in C. elegans.
  • These techniques facilitate deeper insights into lipid metabolism and function in this model organism.
  • The optimized protocols are adaptable for analyzing lipid profiles in other biological samples.