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Updated: Sep 29, 2025
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
A gold nanoparticle-based lateral flow immunoassay for atrazine point-of-care detection using a handhold scanning
Tieqiang Sun1, Zehua Xu1, Shuai Yuan1
1Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment & Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, China.
Abstract:
A method is described to achieve accurate quantitative detection of atrazine (ATZ) in maize by using lateral flow strips based on gold nanoparticles (GNPs) and a handheld scanning reader. GNPs of 15 nm in diameter were applied as label, and a lateral flow immune assay strip was prepared. The linear range was 5.01-95.86 ng mL-1 with a detection limit of 4.92 ng mL-1 in phosphate buffer, 4 times better than the readout by the naked eye. ATZ-spiked corn samples were also analysed. The accuracy of results of spiked samples was confirmed by ELISA and liquid chromatography-tandem mass spectrometry (HPLC), which proved the reliability of the proposed method. A handhold device with an optical scanning system was designed for on-site quantitative detection. Combined with the pretreatment, the assay could be completed in less than 20 min.
