Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Mathematical Model of Wound Healing Incorporating Strain-Induced MSC Differentiation.

Annals of biomedical engineering·2025
Same journal

Correction: Komatsu et al. Three-Dimensional Visualization and Detection of the Pulmonary Venous-Left Atrium Connection Using Artificial Intelligence in Fetal Cardiac Ultrasound Screening. <i>Bioengineering</i> 2026, <i>13</i>, 100.

Bioengineering (Basel, Switzerland)·2026
Same journal

Comparison of CO<sub>2</sub> Laser and Microdebrider in the Surgical Treatment of Pediatric Recurrent Respiratory Papillomatosis: A Retrospective Analysis.

Bioengineering (Basel, Switzerland)·2026
Same journal

Toward More Translational Tumor Models: Breast dECM-Based 3D Systems Capture Native Microenvironmental Cues.

Bioengineering (Basel, Switzerland)·2026
Same journal

Postural Stability Changes During the 4 Phases of the Half Squat: Kinematics Profile of the Center of Pressure and Center of Mass in High-Performance Weightlifters-A Pilot Study.

Bioengineering (Basel, Switzerland)·2026
Same journal

Definite Implant Position as Novel Readout for Effectiveness of Ridge Preservation Indicates to Beneficial Effect of Combined Treatment with Platelet-Rich Fibrin (PRF) and Xenogenic Biomaterial in Bone Regeneration.

Bioengineering (Basel, Switzerland)·2026
Same journal

Trueness and Precision of Intraoral Scanners for 3D-Printed Orthodontic Models with Attachments: An In Vitro Comparative Study.

Bioengineering (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Sep 29, 2025

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells
14:04

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells

Published on: August 1, 2020

6.0K

Modular Bioreactor Design for Directed Tendon/Ligament Tissue Engineering.

Axel J Delakowski1, Jared D Posselt1, Christopher T Wagner1

  • 1Department of Biomedical Engineering, The College of New Jersey, Ewing, NJ 08628, USA.

Bioengineering (Basel, Switzerland)
|March 24, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a novel bioreactor for tissue engineering functional tendons and ligaments using acellular 3D extracellular matrix scaffolds. The system supports strain-induced gene regulation, crucial for cell differentiation and tissue development.

Keywords:
bioreactordifferentiationextracellular matrixligamentmesenchymal cellstendon

More Related Videos

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering
08:04

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering

Published on: April 25, 2013

14.6K
Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model
08:03

Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model

Published on: June 11, 2017

13.8K

Related Experiment Videos

Last Updated: Sep 29, 2025

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells
14:04

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells

Published on: August 1, 2020

6.0K
Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering
08:04

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering

Published on: April 25, 2013

14.6K
Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model
08:03

Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model

Published on: June 11, 2017

13.8K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Reproducible and scalable preparation of functional tissue-engineered tendons and ligaments is challenging.
  • Acellular 3D extracellular matrix (ECM) scaffolds show promise but require optimized culture conditions.
  • Strain-induced gene regulation is critical for tenogenesis and ligamentogenesis.

Purpose of the Study:

  • To evaluate an acellular 3D ECM scaffold for tendon/ligament tissue engineering.
  • To investigate strain-induced gene regulation during mesenchymal stromal cell tenogenesis on ECM scaffolds.
  • To design and verify a novel bioreactor system for controlled mechanical stimulation of 3D scaffolds.

Main Methods:

  • Evaluation of acellular 3D ECM scaffolds for tendon/ligament tissue engineering.
  • Assessment of strain-induced gene regulation in cultured mesenchymal stromal cells.
  • Design and verification of a novel bioreactor using a design control process.
  • Testing of bioreactor functionality for strain (1-10%), frequency (0.2-0.5 Hz), and load (up to 50 N) parameters.

Main Results:

  • Preliminary data revealed unique gene regulation patterns, particularly in Wnt signaling, on 3D ECM scaffolds.
  • A novel bioreactor system was successfully designed and verified to meet critical functional specifications.
  • The bioreactor accommodates clinically-relevant 3D scaffold sizes and allows customizable mechanical strain regimens.
  • Physiological strain levels, frequencies, and accurate load measurements were achieved, demonstrating system reliability.

Conclusions:

  • A novel bioreactor system effectively supports 3D scaffold culture for tendon/ligament tissue engineering.
  • The developed system enables detailed analysis of ECM scaffolds and their influence on cell differentiation.
  • This work provides a model for establishing statistical functionality and reliability in investigative systems for tissue engineering.