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The marginal zone in the human reactive lymph node.
American Journal of Clinical Pathology
|October 1, 1986
Summary
Human lymph nodes contain marginal zone lymphocytes (MZL), similar to the spleen. These cells, identified by sIgM+sIgD- alkaline phosphatase (ALP) activity, vary in distribution within reactive lymph nodes.
Area of Science:
- Immunology
- Cell Biology
- Histopathology
Background:
- The precise location and existence of the marginal zone (MZ) in human lymph nodes remain debated.
- Understanding MZ lymphocyte (MZL) distribution is crucial for comprehending immune responses.
Purpose of the Study:
- To investigate the presence and distribution of marginal zone lymphocytes (MZL) in human reactive lymph nodes.
- To characterize MZL based on their immunophenotype and enzymatic activity.
Main Methods:
- Utilized immuno- and enzyme histochemistry on reactive human lymph nodes and spleens.
- Analyzed the distribution of sIgM+sIgD- MZL expressing alkaline phosphatase (ALP) activity.
Main Results:
- MZL were identified in human reactive lymph nodes, scattered within lymphocytic coronas.
- A distinct population of sIgM+sIgD-ALP+ MZL was found in the outer, loosely arranged rim of zonal-layered coronas.
- The inner rim of coronas comprised sIgM+sIgD+ALP- lymphocytes.
Conclusions:
- Human reactive lymph nodes harbor MZL, analogous to their presence in the spleen.
- MZL represent a dynamic B-cell population whose morphology can range from scattered cells to a discernible peripheral rim.