Related Experiment Video
Updated: Sep 29, 2025
10:31
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
Published on: May 2, 2025
320
Viewing Cortical Collecting Duct Function Through Phenotype-guided Single-Tubule Proteomics.
Nina Himmerkus1, Samuel L Svendsen2, Catarina Quintanova1
1Institute of Physiology, Christian Albrechts University Kiel, Kiel, Germany.
Function (Oxford, England)
|March 25, 2022
Summary
This study links kidney function and protein expression in single mouse kidney tubules. It shows how specific proteins correlate with electrical measurements, aiding in understanding kidney diseases.
Area of Science:
- Nephrology
- Molecular Biology
- Proteomics
Background:
- Kidney heterogeneity complicates omics data integration.
- Single nephron segment analysis is crucial for understanding kidney function.
Purpose of the Study:
- To correlate function and proteome in single mouse cortical collecting ducts (CCDs).
- To investigate the impact of pendrin knockout on CCD proteome and function.
Main Methods:
- Perfused single mouse CCDs in a double-barreled system.
- Recorded electrophysiological parameters (Vte, Rfe, ΔVte(amil)).
- Performed targeted mass spectrometry for proteome analysis.
Main Results:
- Intercalated cell (IC) markers correlated with each other and inversely with principal cell markers.
- Tubular length correlated with actin and Na+-K+-ATPase expression.
- Amiloride-sensitive voltage (ΔVte(amil)) reflected ENaC β-subunit expression and inversely correlated with AQP2 and NEDD4L.
- Pendrin knockout mice showed downregulation of IC markers, potentially explaining salt-losing phenotypes.
Conclusions:
- Functional proteomics on single nephron segments enables robust function-proteome correlations.
- This approach may predict kidney function from omics data.
- Findings offer insights into salt-losing phenotypes observed in pendrin-related kidney disorders.
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