Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

372
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
372
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

6.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
6.3K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

594
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
594
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

10.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
10.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative phase microscopy for time-lapse hypoxia-induced cellular assays based on the transport of intensity equation.

Applied optics·2025
Same author

Tuning Matters: Comparing Lambda Optimization Approaches for Ridge Regression in Genomic Prediction.

Genes·2025
Same author

Mitochondrial morphology in fertile and infertile men: image processing and morphometric analysis of the sperm midpiece.

Frontiers in cell and developmental biology·2025
Same author

Prenatal valproate exposure alters barrel cortex morphology in rats.

Neuroscience letters·2025
Same author

Single-shot 3 × 3 Mueller matrix microscopy with color polarization encoding.

Optics letters·2024
Same author

Refining penalized ridge regression: a novel method for optimizing the regularization parameter in genomic prediction.

G3 (Bethesda, Md.)·2024

Related Experiment Video

Updated: Sep 29, 2025

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.4K

Snapshot polarimetric imaging in multi-view microscopy.

Juan M Llaguno, Federico Lecumberry, Ariel Fernández

    Applied Optics
    |March 25, 2022
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a cost-effective real-time polarimetric microscopy technique. It captures polarization states in a snapshot, enabling dynamic video analysis of microscopic samples.

    More Related Videos

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.0K
    Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
    08:49

    Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

    Published on: December 1, 2023

    1.6K

    Related Experiment Videos

    Last Updated: Sep 29, 2025

    Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
    05:54

    Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

    Published on: September 8, 2023

    1.4K
    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.0K
    Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
    08:49

    Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

    Published on: December 1, 2023

    1.6K

    Area of Science:

    • Optics and Photonics
    • Microscopy
    • Imaging Science

    Background:

    • Polarimetric imaging captures the vector nature of light, with applications in remote sensing and microscopy.
    • Conventional polarimetric microscopy often uses time-division multiplexing, limiting it to static phenomena.

    Purpose of the Study:

    • To develop a cost-effective technique for real-time polarization sensing in microscopy.
    • To enable snapshot acquisition of polarization states for dynamic microscopic imaging.

    Main Methods:

    • A commercial camera was modified with a custom optical system including a microscope objective, lenslet array, and polarization mask.
    • This setup allows for the simultaneous capture of multiple polarization states in a single image (snapshot).

    Main Results:

    • Linear Stokes parameters were obtained in a snapshot, enabling real-time imaging.
    • The proposed scheme demonstrated robustness against misalignment.
    • The technique is suitable for analyzing video sequences of microscopic samples.

    Conclusions:

    • This work presents the first report combining multi-view sensing and polarization imaging for microscopy.
    • The developed technique offers a cost-effective and real-time solution for dynamic polarimetric microscopy.