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Updated: May 3, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Single-shot 3 × 3 Mueller matrix microscopy with color polarization encoding
Abstract:
Snapshot retrieval of linear Stokes parameters in polarized light microscopy has benefited from recent advances in nanotechnology which have made polarization image sensors commercially available. However, real-time retrieval of the 3 × 3 Mueller matrix (MM) is limited by the need for time-sequential change in polarization states in the input. In the present Letter, we propose a cost-effective technique for the real-time retrieval of the 3 × 3 Mueller matrix by encoding linear polarization states in each of the three color channels of the source and analyzing light passing through the sample using a color polarization sensor placed in the imaging plane of a transmission microscope. Validation experiments for samples under real-time imaging conditions are presented. To the best of our knowledge, this is the first report on the snapshot 3 × 3 Mueller matrix element retrieval in polarized light microscopy by combining division of focal plane (DoFP) color polarimetric sensing and polarization color encoding of the source.
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