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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

3.6K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Studying the Cytoskeleton01:17

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The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
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Related Experiment Video

Updated: Sep 29, 2025

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
08:41

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Published on: June 27, 2013

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Force Sensing on Cells and Tissues by Atomic Force Microscopy.

Hatice Holuigue1, Ewelina Lorenc1, Matteo Chighizola1

  • 1CIMAINA and Dipartimento di Fisica "Aldo Pontremoli", Università degli Studi di Milano, Via Celoria 16, 20133 Milan, Italy.

Sensors (Basel, Switzerland)
|March 26, 2022
PubMed
Summary
This summary is machine-generated.

Atomic Force Microscopy (AFM) quantifies the biomechanical properties of the extracellular matrix (ECM) and cells. This technique reveals how mechanical changes in tissues relate to health and disease.

Keywords:
Atomic Force Microscopybiosensorscolloidal probeextracellular matrixglycocalyxmechanobiology

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Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells
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Last Updated: Sep 29, 2025

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Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells
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Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells

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Area of Science:

  • Biophysics
  • Mechanobiology
  • Cell Biology

Background:

  • Physical forces are integral to cellular processes like adhesion, migration, and differentiation.
  • The extracellular matrix (ECM) mechanical properties influence cell fate and tissue development.
  • Understanding force sensing (mechanotransduction) is key to dissecting biological processes.

Purpose of the Study:

  • To apply Atomic Force Microscopy (AFM) for studying biomechanical fingerprints of biological components.
  • To quantitatively characterize physical observables like Young's Modulus (YM) and glycocalyx properties.
  • To correlate mechanical modifications with environmental, pathological, or genetic changes.

Main Methods:

  • Utilizing Atomic Force Microscopy (AFM) for nanoscale force sensing and application.
  • Measuring spatially resolved Young's Modulus (YM) of elasticity for ECM and cells.
  • Characterizing the effective thickness and stiffness of the glycocalyx.

Main Results:

  • AFM successfully quantified biomechanical fingerprints of ECM, cells, nucleus, lamellipodia, and glycocalyx.
  • Young's Modulus (YM) of elasticity was spatially resolved for ECM and cells.
  • Effective thickness and stiffness of the glycocalyx were quantitatively characterized.

Conclusions:

  • AFM is an enabling technology for biophysics and mechanobiology research.
  • Quantitative biomechanical characterization by AFM can be correlated with physiological and pathological conditions.
  • AFM provides insights into how mechanical properties influence biological systems.