Separation of gallic acid from Cornus officinalis and its interactions with corn starch

D101 macroporous resin combined with high speed counter-current chromatography (D101 MR-HSCCC) was used to separate gallic acid (GA) from Cornus officinalis, and GA was added to starch-based products to improve food quality. The interaction and action mechanism of corn starch (CS) with GA were investigated for prediction and thereby controlling the structure and functions of starch-based foods. Results show that GA with 98.72% purity was successfully obtained using the D101 MR-HSCCC technique. HSCCC solvent system was composed of n-hexane-ethyl acetate-methanol-water (1:5:1:5, v/v/v/v). GA inhibited CS dissolution and improved CS swelling. Based on the particle size distribution, GA could enlarge the size of CS-GA complexes. FT-IR spectra exhibit that the interactions between CS and GA may comprise the intermolecular hydrogen bond and non-covalent bond. The results of XRD, LF-NMR and AFM show that the presence of GA could increase the relative crystallinity of CS, decrease the spin relaxation time (T₂)_, and change the surface morphology of CS via the modification of hydrogen bonds distribution. Finally, SEM analysis indicates that GA could change the microstructure of CS-GA complexes. These findings facilitate the development of CS-based products and utilization of CS.