Synthetic estrogen bioaccumulates and changes the behavior and biochemical biomarkers in adult zebrafish

Estrogen is considered to be an endocrine disrupter and is becoming increasingly more prevalent in the daily life of humans. In some cases, estrogen is not fully metabolized by organisms and may be excreted in either its original form or in organic complex forms, into water residue systems reaching concentrations of 0.05 ng.L^-1 to 75 ng.L^-1. However, estrogen 17α-ethinylestradiol (EE2), which is used in oral contraceptives, is very difficult to remove from water. Here, we evaluated whether the synthetic hormone, EE2, affects the nervous system and the behavior of adult zebrafish. We established a range of concentrations (0.05, 0.5, 5, 50, and 75 ng.L^-1), in addition to the control, to evaluate the effect of this compound and its bioaccumulation in zebrafish tissues. Here we show that EE2 bioaccumulates in fish and can change its behavior with an increased time in the upper zone (novel tank test) and far from the shoal segment (social preference test), demonstrating a clear anxiolytic pattern. The anxiolytic effect of EE2 can be harmful as it can affect the stress response of the species. The results presented herein reinforce the idea that the presence of EE2 in environmental water can be dangerous for non-target animals.