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Complete Genome Sequence of Burkholderia cenocepacia Phage Paku

Sefanit Rezene1, Guichun Yao2, Tram Le1,2

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|March 28, 2022
PubMed

Insights

The Burkholderia cenocepacia phage Paku genome was analyzed, revealing a phiKMV-like arrangement. This phage may possess a temperate lifestyle, which is unusual for its family.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Burkholderia cenocepacia is a significant opportunistic pathogen, particularly in individuals with cystic fibrosis.
  • Bacteriophages, viruses that infect bacteria, are increasingly studied for their potential roles in bacterial pathogenesis and control.

Purpose of the Study:

  • To characterize the genome of Burkholderia cenocepacia phage Paku.
  • To investigate the genomic features of phage Paku and infer its lifestyle.

Main Methods:

  • Whole-genome sequencing of phage Paku.
  • Bioinformatic analysis of the phage genome, including identification of structural and functional genes.
  • Comparative genomic analysis with related phages.

Main Results:

  • The genome of phage Paku is 42,727 base pairs long and exhibits a phiKMV-like genome arrangement.
  • Identification of genes encoding T7-like tail components.
  • Presence of a tyrosine integrase gene, suggesting a potential temperate lifestyle.

Conclusions:

  • Phage Paku possesses genomic characteristics atypical for the Autographiviridae family.
  • The findings suggest that phage Paku may exhibit a temperate lifestyle, which warrants further investigation into its biological behavior and implications for Burkholderia cenocepacia infections.

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