Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Palette of Minimally Tagged Sucrose Analogues for Real-Time Raman Imaging of Intracellular Plant Metabolism.

Angewandte Chemie (Weinheim an der Bergstrasse, Germany)·2024

Same author

Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques.

Methods in molecular biology (Clifton, N.J.)·2024

Same author

A Palette of Minimally Tagged Sucrose Analogues for Real-Time Raman Imaging of Intracellular Plant Metabolism.

Angewandte Chemie (International ed. in English)·2021

Same author

Measuring Phloem Transport Velocity in Arabidopsis Seedlings Using the Fluorescent Coumarin Glucoside, Esculin.

Methods in molecular biology (Clifton, N.J.)·2019

Same author

The Coumarin Glucoside, Esculin, Reveals Rapid Changes in Phloem-Transport Velocity in Response to Environmental Cues.

Plant physiology·2018

Same author

Illuminating the translocation stream.

Current opinion in plant biology·2018

Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 28, 2025

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

Super-Resolution Imaging of Plasmodesmata Using 3D Structured Illumination Microscopy.

¹The School of Life Sciences, University of Glasgow, Glasgow, UK. Kirsten.Knox@glasgow.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|March 29, 2022

Summary

This summary is machine-generated.

Imaging plasmodesmata (PD) in live plant cells is challenging due to their small size. This study presents a 3D-SIM method for visualizing PD internal structures in living BY2 cells.

Keywords:

3D-SIM BY2 Desmotubule Fluorescence Plasmodesmata Super-resolution

More Related Videos

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

Related Experiment Videos

Last Updated: Sep 28, 2025

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

Area of Science:

Plant cell biology
Microscopy techniques

Background:

Plasmodesmata (PD) are crucial for plant cell-to-cell communication.
Their small size (30-50 nm) limits analysis by conventional light microscopy.
Resolving internal structures like the desmotubule is impossible with standard light microscopy.

Purpose of the Study:

To develop and present a method for imaging plasmodesmata in live plant cells.
To overcome the resolution limitations of light microscopy for PD analysis.
To enable visualization of internal PD structures in living cells.

Main Methods:

Utilized 3D structured illumination microscopy (3D-SIM).
Optimized imaging protocols for live BY2 plant cells.
Applied super-resolution microscopy to overcome optical resolution limits.

Main Results:

Successfully imaged plasmodesmata in live BY2 cells using 3D-SIM.
Achieved enhanced lateral and axial resolution beyond the diffraction limit.
Demonstrated the feasibility of visualizing PD in a living cell context.

Conclusions:

3D-SIM provides a viable method for high-resolution imaging of plasmodesmata in live plant cells.
This technique allows for the study of dynamic PD processes.
The presented method advances the understanding of cell-to-cell communication in plants.