Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endotoxin-induced changes in nitrogen metabolism.

L Kilpatrick-Smith, M C Yoder, R A Polin

    Research Communications in Chemical Pathology and Pharmacology
    |September 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genotoxic stresses promote clonal expansion of hematopoietic stem cells expressing mutant p53.

    Leukemia·2017
    Same author

    Endothelial colony-forming cells and pro-angiogenic cells: clarifying definitions and their potential role in mitigating acute kidney injury.

    Acta physiologica (Oxford, England)·2017
    Same author

    Phosphatase PRL2 promotes AML1-ETO-induced acute myeloid leukemia.

    Leukemia·2017
    Same author

    Infant mortality in the United States.

    Journal of perinatology : official journal of the California Perinatal Association·2016
    Same author

    Early radiologic evidence of severe respiratory distress syndrome as a predictor of nasal continuous positive airway pressure failure in extremely low birth weight newborns.

    Journal of perinatology : official journal of the California Perinatal Association·2014
    Same author

    Isolating and defining cells to engineer human blood vessels.

    Cell proliferation·2011
    Same journal

    Is heat acclimation able to increase whole-body sensitivity to insulin?

    Research communications in chemical pathology and pharmacology·1994
    Same journal

    Vasodilator effects of clonidine on the mesenteric arterial beds in normotensive and spontaneously hypertensive rats.

    Research communications in chemical pathology and pharmacology·1994
    Same journal

    Elevation of ceruloplasmin activity involved in changes of hepatic metal concentration in primary biliary cirrhosis.

    Research communications in chemical pathology and pharmacology·1994
    Same journal

    Copper cytotoxicity impairs DNA synthesis but not protein phosphorylation upon growth stimulation in LEC mutant rat.

    Research communications in chemical pathology and pharmacology·1994
    Same journal

    Enhanced sensitivity of mdx mice to intramuscular injection of compound 48/80.

    Research communications in chemical pathology and pharmacology·1994
    Same journal

    Inhibition of antigen-induced airway hyperresponsiveness in rats: effects of ozagrel (a thromboxane A2 synthase inhibitor) and of CV-3988 (a platelet activating factor antagonist).

    Research communications in chemical pathology and pharmacology·1994
    See all related articles

    Early endotoxemia in rats impairs nitrogen metabolism, increasing plasma ammonia and urea. Liver ureagenesis and amino acid levels rise, while energy charge and pyridine nucleotide balance are disrupted.

    Area of Science:

    • Biochemistry
    • Physiology
    • Toxicology

    Background:

    • Endotoxemia, often caused by Gram-negative bacteria like E. coli, can lead to significant physiological disturbances.
    • Understanding the early metabolic alterations during endotoxemia is crucial for developing timely interventions.

    Purpose of the Study:

    • To investigate the immediate effects of a sublethal dose of E. coli endotoxin on nitrogen metabolism and energy balance in rats.
    • To identify early biochemical markers of endotoxemia.

    Main Methods:

    • Rats were administered a sublethal dose of E. coli endotoxin intraperitoneally after fasting.
    • Plasma and liver samples were collected after 12 hours to measure levels of ammonia, urea, amino acids, glucose, ketones, and hepatic energy charge.
    • Mitochondrial and cytosolic pyridine nucleotide redox states were assessed.

    Related Experiment Videos

    Main Results:

    • Endotoxin administration led to increased plasma ammonia, urea, glutamine, glutamate, and aspartate within 12 hours.
    • Hepatic ureagenesis and levels of glutamate, glutamine, aspartate, alanine, and asparagine were elevated.
    • Liver glucose and ketone levels remained unchanged, but hepatic ATP/ADP ratios decreased, indicating altered energy status.

    Conclusions:

    • Early endotoxemia significantly disrupts nitrogen metabolism, characterized by increased ammonia and urea production and altered amino acid profiles.
    • Mitochondrial pyridine nucleotide oxidation and cytosolic pyridine nucleotide reduction suggest impaired cellular energy metabolism during early endotoxemia.
    • These metabolic disturbances represent early effects of endotoxemia, highlighting potential targets for therapeutic strategies.