Related Experiment Video
Updated: Sep 28, 2025
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
The direct application of plasma droplet digital PCR in the ultra-early pathogen detection and warning during sepsis:
Ke Lin1, Hao-Cheng Zhang1, Yuan-Han Zhao1
1Department of Infectious Disease of Huashan Hospital, National Medical Center for Infectious Diseases and Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, Fudan University, Shanghai 200040, China.
Background:
Sepsis is a severe and acute disease associated with high mortality, for those who survive, long-term morbidity. In-time detection of pathogen for proper and optimized treatment in the early stage of sepsis is crucial.
Methods:
We performed droplet digital PCR (ddPCR) on two cases of sepsis. The clinical information, laboratory test results, and the therapeutic regimen was detailed recorded.
Results:
ddPCR showed its clinical value of sepsis ultra-early diagnosis and the guidance of medication in both cases we provided.
Conclusions:
ddPCR has potential for rapid and precise diagnose in ultra-early stage of sepsis for its high sensitivity and short turn-around-time, thus benefiting in optimal and timely treatment of sepsis.
