Related Experiment Video
Updated: Sep 28, 2025
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
Comprehensive comparative analysis of lipid profile in dried and fresh walnut kernels by UHPLC-Q-Exactive Orbitrap/MS
Ping Wang1, Linlin Zhong2, Hongbin Yang2
1Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China; Production & Construction Group Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang, Alar, Xinjiang 843300, PR China.
Abstract:
Lipids are important nutrient components in walnuts. However, there has been a lack of comprehensive analysis of lipid composition in walnuts. Here, UHPLC-Q-Exactive Orbitrap/MS was employed to qualify and quantify the lipid compounds in seven walnut varieties from Xinjiang. A total of 390 lipids were tentatively identified, which were classified into six categories and 30 subcategories. GL and GP were the two most abundant lipids in all walnuts. Compared with fresh walnuts (FW), dried walnuts (DW) showed significantly higher lipids; in the 30 subcategories, DW showed decreases in some MGDG and PC while increases in LPC and MePC, all of which were related to the moisture content of walnuts. Totally, 128 lipids can be used as potential markers to distinguish FW from DW. Besides, the lipid profile varied among different varieties of walnut kernels. These findings may facilitate walnut variety selection and processing based on the characteristic lipids.
More Related Videos
08:56Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
08:27Large-Scale Multi-Omics Genome-Wide Association Studies Mo-GWAS: Guidelines for Sample Preparation and Normalization
Published on: July 27, 2021