LncRNA CALML3-AS1 suppresses papillary thyroid cancer progression via sponging miR-20a-5p/RBM38 axis

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Updated: Sep 28, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.5K

Xiaozhou Zhang1,2, Xiaojian Zhang2, Qian Jia3

  • 1Department of thyroid surgery, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, 450000, Henan Province, China.

BMC Cancer
|March 30, 2022
PubMed
Abstract

Insights

Long non-coding RNA CALML3-AS1 (CALML3-AS1) acts as a tumor suppressor in papillary thyroid cancer (PTC) by regulating the miR-20a-5p/RBM38 axis. CALML3-AS1 overexpression inhibits PTC progression and serves as a positive prognostic marker.

Area of Science:

  • Molecular Oncology
  • Cancer Biology

Background:

  • Thyroid cancer (TC) incidence and mortality are rising globally.
  • Understanding TC molecular mechanisms and identifying therapeutic targets is crucial.

Purpose of the Study:

  • To investigate the role of lncRNA CALML3-AS1 in papillary thyroid cancer (PTC) development.
  • To elucidate the molecular mechanisms involving CALML3-AS1 in PTC.

Main Methods:

  • qRT-PCR and Western blot to assess gene expression in PTC tissues and cell lines.
  • Cell proliferation, migration, invasion, and apoptosis assays to evaluate CALML3-AS1 function.
  • RNA pull-down and luciferase assays to confirm molecular interactions.

Main Results:

  • CALML3-AS1 and RBM38 expression were significantly downregulated in PTC.
  • miR-20a-5p expression was significantly upregulated in PTC.
  • CALML3-AS1 overexpression inhibited PTC cell proliferation, migration, and invasion.
  • CALML3-AS1 acts as a ceRNA for miR-20a-5p, suppressing RBM38 expression.

Conclusions:

  • CALML3-AS1 functions as a ceRNA for miR-20a-5p, regulating RBM38 expression in PTC.
  • CALML3-AS1 overexpression inhibits PTC progression.
  • CALML3-AS1 is a potential prognostic biomarker for PTC.
  • Targeting the CALML3-AS1/miR-20a-5p/RBM38 axis offers a novel therapeutic strategy for PTC.
Keywords:
Papillary thyroid cancerRBM38lncRNAlncRNA CALML3-AS1miR-20a-5p

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