Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Conversion02:08

Gene Conversion

10.0K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
10.0K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

15.2K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
15.2K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.6K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.6K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

13.0K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
13.0K
Next-generation Sequencing03:00

Next-generation Sequencing

93.2K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
93.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On-DNA Epoxide Synthesis and Ring-Opening Derivatization.

Organic letters·2026
Same author

DNA-Compatible Synthesis of β-Ketoamides as Intermediates for On-DNA Chemical Diversification.

Organic letters·2026
Same author

Accessing α-Ketoamides on DNA through a Coupling-Oxidation Strategy.

Organic letters·2026
Same author

Selenoxides unlock C-H bonds of biomacromolecules.

Nature chemistry·2025
Same author

DNA-Compatible Synthesis of Pyrrolidine-Fused Scaffolds via a One-Pot Three-Component Cycloaddition Strategy.

Organic letters·2025
Same author

Recent advances in DNA-encoded libraries.

Chemical communications (Cambridge, England)·2025

Related Experiment Video

Updated: Sep 28, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.4K

Switchable DNA-Encoded Chemical Library: Interconversion between Double- and Single-Stranded DNA Formats.

Guixian Zhao1, Xiaohong Fan1,2, Yangfeng Li1,3

  • 1Chongqing Key Laboratory of Natural Product Synthesis and, Drug Research, Innovative Drug Research Center, School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331, P. R. China.

Chembiochem : a European Journal of Chemical Biology
|March 30, 2022
PubMed
Summary
This summary is machine-generated.

DNA-encoded chemical libraries (DEL) offer vast hit discovery potential. A novel reversible covalent headpiece (RCHP) design enables seamless interconversion between single- and double-stranded DEL formats for enhanced flexibility.

Keywords:
DNADNA-encoded chemical librarydrug discoveryphoto-crosslinkingselection

More Related Videos

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.2K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.5K

Related Experiment Videos

Last Updated: Sep 28, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.4K
Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.2K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.5K

Area of Science:

  • Medicinal Chemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • DNA-encoded chemical libraries (DEL) are powerful tools for hit discovery in pharmaceuticals and academia.
  • Current DEL encoding strategies are typically based on either single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA).
  • These ssDNA and dsDNA formats possess distinct advantages but are generally incompatible, limiting their combined application.

Purpose of the Study:

  • To introduce a novel method for interconverting between ssDNA and dsDNA DEL formats.
  • To present the "reversible covalent headpiece (RCHP)" design for flexible DEL construction.
  • To enhance the adaptability and utility of DEL technology in drug discovery.

Main Methods:

  • Development of the "reversible covalent headpiece (RCHP)" strategy.
  • Demonstration of interconversion between ssDNA and dsDNA formats using the RCHP design.
  • Evaluation of the robustness of synthesis and flexibility of applications.

Main Results:

  • The RCHP design facilitates robust synthesis of DELs.
  • The RCHP enables facile and reversible conversion between ssDNA and dsDNA DEL formats.
  • This interconversion strategy enhances the flexibility of DEL applications in various experimental setups.

Conclusions:

  • The RCHP design bridges the incompatibility gap between ssDNA and dsDNA DEL formats.
  • This innovation offers a versatile platform for DEL construction and application.
  • Future opportunities exist to advance DEL technology into a comprehensive drug discovery platform.