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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

14.6K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
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A compact microfluidic geometry for multiplexing enzyme-linked immunosorbent assays.

Basant Giri1, Debashis Dutta1

  • 1Department of Chemistry, University of Wyoming, Laramie, Wyoming, USA.

Electrophoresis
|March 31, 2022
PubMed
Summary
This summary is machine-generated.

This study miniaturizes multiplex enzyme-linked immunosorbent assay (ELISA) using a circular microchannel design, reducing size and sample volume. The new method enhances assay capabilities through electrokinetic preconcentration.

Keywords:
ELISAdiffusionelectroosmotic flowmicrofluidicmultiplex

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Multiplex enzyme-linked immunosorbent assay (ELISA) is a powerful tool for detecting multiple analytes simultaneously.
  • Previous work demonstrated multiplex ELISA in microchannels exploiting diffusion-based product spread.
  • Existing methods often require larger sample volumes and footprint areas.

Purpose of the Study:

  • To develop a miniaturized multiplex ELISA platform with a reduced footprint and sample volume.
  • To implement a circular microchannel layout for enhanced assay integration.
  • To explore electroosmosis for selective assay segment coating and analyte preconcentration.

Main Methods:

  • A circular microchannel design was fabricated to arrange assay segments circumferentially.
  • Multiplex cytokine detection was performed using a 5-plex assay.
  • Electroosmosis was employed for selective coating of assay segments with target molecules.
  • Analyte preconcentration using electric fields was investigated.

Main Results:

  • A 5-plex cytokine ELISA was successfully demonstrated within a 1.5 cm x 1.5 cm area, a threefold reduction in footprint.
  • The use of electroosmosis for coating enabled shorter cross-sectional channel dimensions.
  • Electrokinetic preconcentration offers potential for enhanced assay sensitivity.

Conclusions:

  • The circular microchannel multiplex ELISA significantly reduces assay footprint and sample volume requirements.
  • Electroosmosis provides a versatile method for selective coating and enables integration of advanced functionalities like electrokinetic preconcentration.
  • This miniaturized platform holds promise for high-throughput, low-volume diagnostic applications.