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Bioactive Decellularized Extracellular Matrix Hydrogel Microspheres Fabricated Using a Temperature-Controlling

Zudong Lin1, Zilong Rao2, Jiaxin Chen2

  • 1PCFM Lab, GD HPPC Lab, School of Chemistry, Sun Yat-sen University, 132 Waihuan West Road, HEMC, Guangzhou 510006, China.

ACS Biomaterials Science & Engineering
|March 31, 2022
PubMed
Summary

Researchers developed decellularized extracellular matrix (dECM) hydrogel microspheres using a novel microfluidic system. These bioactive microspheres support cell growth and show promise for tissue regeneration and biomedical applications.

Keywords:
Schwann celldecellularized extracellular matrixhydrogel microspheresmicrofluidicsneural stem/progenitor cell

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Microfluidics

Background:

  • Hydrogel microspheres are valuable 3D microcarriers for cell culture and therapies.
  • Decellularized extracellular matrix (dECM) hydrogels retain native tissue cues, enhancing bioactivity and promoting regeneration.
  • Existing methods for dECM hydrogel production can be limited in efficiency and scalability.

Purpose of the Study:

  • To develop a high-throughput method for producing pristine decellularized extracellular matrix (dECM) hydrogel microspheres.
  • To characterize the physical properties and stability of the dECM hydrogel microspheres.
  • To evaluate the cytocompatibility and cell-promoting capabilities of the dECM hydrogel microspheres for neural cells.

Main Methods:

  • A novel two-stage temperature-controlling microfluidic system was employed for microsphere fabrication.
  • Porcine decellularized peripheral nerve matrix (pDNM) was used as the dECM source, processed without additional crosslinkers.
  • Microsphere size was controlled by adjusting water/oil phase ratios.
  • Cell viability, adhesion, proliferation, and extension were assessed using Schwann cells, PC12 cells, and neural stem/progenitor cells.

Main Results:

  • The microfluidic system enabled high-throughput production of stable pDNM microspheres (pDNM-MSs) with controlled sizes.
  • pDNM-MSs maintained spherical shape and nanofibrous ultrastructure for over 14 days.
  • Cells cultured on pDNM-MSs exhibited excellent viability, adhesion, and enhanced cell extension compared to gelatin microspheres.
  • Neural stem/progenitor cells showed robust attachment and facilitated proliferation on pDNM-MSs.

Conclusions:

  • The developed microfluidic system efficiently produces stable and bioactive dECM hydrogel microspheres.
  • These pDNM-MSs provide a superior microenvironment for 3D cell culture, promoting neural cell growth and function.
  • The dECM hydrogel microspheres hold significant potential for diverse biomedical applications, including regenerative medicine.