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Related Experiment Video

Updated: Mar 25, 2026

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
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Decellularized Dental Pulp Matrix Hydrogel Promotes Functional Endodontic Regeneration In Situ.

Zelin Liang1, Yuwen Feng1, Sien Zhang1

  • 1Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, China.

International Endodontic Journal
|March 24, 2026
PubMed
Summary

Porcine-derived decellularized dental pulp matrix hydrogel (pDDPM-G) shows promise for regenerative endodontics. This scaffold material successfully regenerated the pulp-dentine complex in dogs, promoting dentinogenesis, angiogenesis, and neurogenesis.

Keywords:
angiogenesisdentinogenesisendodontics regenerationextracellular matrixhydrogelneurogenesis

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Endodontics
  • Tissue Engineering

Background:

  • Effective scaffold materials are essential for successful regenerative endodontic therapies.
  • Porcine-derived decellularized dental pulp matrix hydrogel (pDDPM-G) was developed as a novel scaffold.
  • The study aimed to evaluate pDDPM-G for in situ regeneration of the pulp-dentine complex.

Purpose of the Study:

  • To assess the efficacy of pDDPM-G as a scaffold in regenerative endodontics.
  • To investigate the role of pDDPM-G in pulp-dentine complex regeneration in a canine model.
  • To compare pDDPM-G with conventional treatments and assess its impact on mineralization, vascularization, and innervation.

Main Methods:

  • An orthotopic pulp regeneration model was established in beagle dogs.
  • pDDPM-G was applied directly or as a carrier for dental pulp stem cells (DPSCs).
  • Histological, immunofluorescence, and CT imaging analyses were performed at 14 and 90 days post-surgery.

Main Results:

  • pDDPM-G was critical for regenerative endodontic procedures, whether with DPSC transplantation, blood induction (BI), or alone.
  • pDDPM-G reduced intracanal calcification compared to conventional revascularization therapy (BI) alone.
  • Significantly higher expression of odontoblast-like, angiogenic, and neurogenic markers was observed in pDDPM-G groups.

Conclusions:

  • pDDPM-G is a significant biomaterial for functional endodontic regeneration in an orthotopic canine model.
  • pDDPM-G facilitates root development, pulp-like tissue formation, dentinogenesis, angiogenesis, and neurogenesis.
  • This scaffold enables the reconstruction of hierarchically distributed functional pulp-dentine complexes.