Bioaugmentation for low C/N ratio wastewater treatment by combining endogenous partial denitrification (EPD) and...

Endogenous partial denitrification (EPD) and denitrifying phosphorous removal (DPR) were combined in a novel A²/O - MBBR (Anaerobic Anoxic Oxic - Moving Bed Biofilm Reactor) system for low carbon/nitrogen (C/N) ratio wastewater treatment. The DPR performance was compared and the nutrient metabolism was elucidated based on the optimization of hydraulic retention time (HRT, 4-12 h) and nitrate recycling (R, 200%-600%). In the continuous-flow, the nitrate (NO₃^-) denitrification accompanied by nitrite (NO₂^-, via EPD) accumulation with the nitrate-to-nitrite transformation ratio (NTR) of 35.87%-43.31% in the anoxic zones. At HRT of 12 h with R of 500%, batch test initially revealed the DPR mechanism using both NO₃^- and NO₂^- as electron acceptor, where denitrifying phosphorus accumulation organisms (DPAOs) and denitrifying glycogen accumulation organisms (DGAOs) were the main contributors for EPD with incomplete denitrification (NO₃^- → NO₂^-). Furthermore, stoichiometry-based functional bacteria analysis displayed that higher bioactivity of DPAOs (NO₂^-→N₂, 57.30%; NO₃^-→N₂, 35.85%) over DGAOs (NO₃^-→N₂, 6.85%) facilitated the anoxic NO₃^- reduction. Microbial community analysis suggested that Cluster I of Defluviicoccus-GAO group (∼4%) was responsible for stable NO₂^- accumulation performance via EPD, while increased Accumulibacter-PAO group (by ∼15%) contributed to the advanced nutrient removal. Based on the achievement of NO₂^- accumulation, the application feasibility of integrated EPD - DPR - Anammox for deep-level nutrient removal was discussed.