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Related Experiment Video

Updated: Sep 28, 2025

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Published on: June 11, 2016

Precursor Quantitation Methods for Next Generation Food Production.

Xinran Wang¹, Xiaozhou Luo^1,2

¹CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Frontiers in Bioengineering and Biotechnology

|April 1, 2022

Summary

This summary is machine-generated.

Synthetic biology offers sustainable food alternatives by engineering microbes. Quantifying metabolic precursors guides pathway engineering for efficient production of healthy food components.

Keywords:

food method precursor quantitation synthetic biology

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Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

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Last Updated: Sep 28, 2025

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

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Published on: June 11, 2016

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn Zea mays As an Exemplar

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Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

Published on: September 20, 2016

Area of Science:

Biotechnology
Metabolic Engineering
Food Science

Background:

Traditional agriculture struggles to meet rising global food demand sustainably.
Growing consumer demand for natural, healthy food additives drives innovation.
Synthetic biology presents a promising alternative for food component production.

Purpose of the Study:

To review methods for quantifying cellular biosynthetic precursors in engineered microbes.
To guide precise metabolic pathway engineering for food component synthesis.
To assess the advantages and limitations of various quantification techniques.

Main Methods:

Literature review of precursor quantification methods.
Analysis of techniques for energy molecules and redox co-factors.
Discussion of methods applicable to microbial cell factories.

Main Results:

Summarized various quantification methods for key biosynthetic precursors.
Highlighted the importance of precursor quantitation for metabolic flux analysis.
Provided insights into engineering microbial pathways for food production.

Conclusions:

Quantifying metabolic precursors is crucial for optimizing synthetic biology approaches in food production.
Engineered microbial cell factories offer a sustainable route to novel food alternatives and additives.
This review aids researchers in selecting appropriate methods for pathway engineering.