Related Experiment Video
Updated: Sep 28, 2025
13:10
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
Published on: March 10, 2020
7.4K
Circular RNA circStag1 promotes bone regeneration by interacting with HuR
Gaoyang Chen1,2, Canling Long1,2, Shang Wang1,2
1Department of Spine Surgery and Institute for Orthopaedic Research, the Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen Key Laboratory of Musculoskeletal Tissue Reconstruction and Function Restoration, Shenzhen, 518020, China.
Bone Research
|April 1, 2022
Summary
Circular RNAs (circRNAs) like circStag1 are crucial for bone health. Downregulated circStag1 in osteoporosis suggests it can promote bone regeneration via the HuR/Wnt pathway, offering new therapeutic strategies.
Area of Science:
- Biochemistry
- Molecular Biology
- Orthopedics
Background:
- Postmenopausal osteoporosis is a metabolic bone disorder increasing fracture risk.
- Circular RNAs (circRNAs) regulate bone metabolism, but their role in osteoporosis is unclear.
- Osteoporosis involves bone microarchitecture deterioration.
Purpose of the Study:
- Investigate the function of circRNAs in postmenopausal osteoporosis.
- Identify specific circRNAs involved in bone metabolism regulation.
- Explore the molecular mechanisms underlying circRNA action in osteoporosis.
Main Methods:
- Quantified circStag1 expression in osteoporotic bone marrow mesenchymal stem cells (BMSCs) and patient samples.
- Overexpressed circStag1 in BMSCs and assessed osteogenic capability.
- Investigated circStag1 interaction with Human Antigen R (HuR) and its effect on Wnt signaling.
- Utilized circStag1-loaded adeno-associated virus (circStag1-AAV) in an ovariectomized rat model.
Main Results:
- circStag1 expression was significantly downregulated in osteoporotic BMSCs and tissues.
- Overexpression of circStag1 enhanced BMSC osteogenic differentiation.
- circStag1 promoted HuR cytoplasmic translocation, activating the Wnt pathway by stabilizing Lrp5/6 and β-catenin.
- In vivo circStag1-AAV administration promoted bone formation and prevented bone loss in rats.
Conclusions:
- circStag1 is a critical regulator of bone metabolism and osteogenesis.
- circStag1 promotes bone regeneration by modulating the HuR/Wnt signaling pathway.
- circStag1 represents a potential therapeutic target for postmenopausal osteoporosis.
Related Concept Videos
02:37Restarting Stalled Replication Forks
5.9K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.9K
01:40Bone Remodeling
38.6K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.6K
01:31Osteoclasts in Bone Remodeling
3.3K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.3K
01:16TGF - β Signaling Pathway
7.8K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.8K
01:17Hormones and Bone Tissue
3.0K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
3.0K
02:55The Nucleolus
9.3K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
9.3K