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Electrochemically switchable and tunable luciferase bioluminescence.

Ali Othman1, Evgeny Katz1, Oleh Smutok1

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Summary

Electrochemical signals were used to reversibly control the bioluminescence of immobilized luciferase. Applying a reductive potential depleted oxygen, inhibiting the enzyme, while releasing the potential reactivated it.

Keywords:
BioluminescenceElectrochemically switchableElectrochemically tunableLuciferaseModified electrode

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Area of Science:

  • Electrochemistry
  • Biocatalysis
  • Enzyme immobilization

Background:

  • Luciferase requires oxygen for biocatalytic activity.
  • Electrode-immobilized enzymes offer platforms for electrochemical biosensing.
  • Controlling enzyme activity with external stimuli is crucial for advanced applications.

Purpose of the Study:

  • To demonstrate reversible control of electrode-immobilized luciferase activity using electrochemical signals.
  • To investigate the mechanism of oxygen depletion-induced enzyme inhibition.
  • To establish a method for switching bioluminescence on and off.

Main Methods:

  • Immobilizing luciferase onto an electrode surface.
  • Applying a reductive potential (-0.9 V vs. Ag/AgCl) to consume oxygen near the electrode.
  • Monitoring bioluminescence changes in response to applied potentials.
  • Analyzing the effect of oxygen concentration on enzyme activity.

Main Results:

  • Biocatalytic activity and bioluminescence of immobilized luciferase were reversibly tuned by electrochemical signals.
  • A reductive potential led to oxygen depletion and subsequent luciferase inhibition.
  • Releasing the potential allowed oxygen diffusion, reactivating the luciferase.
  • Cyclic potential application and release resulted in reversible inhibition-activation.

Conclusions:

  • Electrochemical control of immobilized luciferase activity is feasible.
  • Oxygen depletion is an effective mechanism for reversible enzyme inhibition.
  • This work provides a foundation for electrochemically switchable bioluminescent systems.