Related Experiment Video
Updated: Sep 28, 2025
Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
Published on: July 10, 2021
Follicular fluid supplemented with copper and zinc increase the maturation rate of mouse vitrified-warmed oocytes
S Geravandi1, A Karami2, M Azadbakht3
1Department of Biology, Faculty of Basic Sciences, Razi University, Taq-e Bostan, Bagh-e Abrisham, 6714967346, Kermanshah, Iran.
Background:
Immature oocyte cryopreservation is a therapeutic option in assisted reproductive technology.
Objective:
To evaluate the effects of supplementing oocyte maturation medium with human follicular fluid (hFF), zinc and copper.
Materials And Methods:
Four different maturation media supplemented with 10% follicular fluid, 4 μg/mL copper and 1 μg/mL zinc were used for vitrified-warmed oocytes of mouse.
Results:
Maturation rate was the highest (63.3%) in the presence of zinc. Cleavage and blastocyst rates in groups with copper and zinc were significantly higher than the control group (39.9% and 46.4% vs. 28.8%), without any significant difference between Zn and Cu.
Conclusion:
Our findings indicate the high importance of using hFF as a natural medium, and also zinc and copper as two efficient trace elements in the maturation medium for vitrified-warmed oocytes.
