Related Experiment Video
Updated: Sep 28, 2025
06:28
E-Patient Counseling Trial E-PACO: Computer Based Education versus Nurse Counseling for Patients to Prepare for Colonoscopy
Published on: August 1, 2019
8.4K
A Prospective, Double-Blind, Randomized, Placebo-Controlled, Crossover Study Using an Orally Administered Oxalate
Emily Quintero1, Victoria Yvonne Bird2, Howard Liu3
1University of Florida College of Medicine, Gainesville, Florida.
Kidney360
|April 4, 2022
Summary
An oral oxalate decarboxylase (OxDC) significantly reduced urinary oxalate levels by 29% in healthy subjects on a high-oxalate diet. This intervention demonstrated effectiveness without adverse events, offering a potential strategy for managing hyperoxaluria.
Area of Science:
- Nephrology
- Gastroenterology
- Biochemistry
Background:
- Hyperoxaluria, linked to kidney stones and ESKD, stems from dietary oxalate, low calcium, or endogenous overproduction.
- This study investigates an acid-stable oxalate decarboxylase (OxDC) for reducing urinary oxalate.
Purpose of the Study:
- To evaluate the efficacy of orally administered OxDC in decreasing urinary oxalate excretion.
- To assess the safety and impact of OxDC on other urinary parameters in healthy volunteers.
Main Methods:
- A prospective, double-blind, randomized, placebo-controlled, crossover trial involving 33 healthy volunteers.
- Subjects consumed a high-oxalate diet (750-800 mg/day) and received OxDC or placebo three times daily for 4 days, with a 2-day washout period.
- Six 24-hour urine collections were analyzed for oxalate and other solutes.
Main Results:
- OxDC treatment led to a significant mean reduction of 29% (12.5 mg) in 24-hour urinary oxalate excretion (P<0.001).
- OxDC was effective in 94% of subjects and reduced urinary oxalate by 24% compared to placebo (P<0.001).
- No significant changes were observed in urinary creatinine, uric acid, citrate, magnesium, or calcium levels.
Conclusions:
- Orally administered OxDC effectively reduces urinary oxalate in healthy individuals on a high-oxalate diet.
- OxDC treatment is safe and does not alter key urinary parameters related to calcium oxalate supersaturation.
Keywords:
Carboxy-Lyaseschronic kidney diseasecross-over studiesdouble-blind methodhyperoxaluriakidney stonenephrolithiasisoxalateoxalate decarboxylaseoxalate nephrophathyoxalate-rich foodsprospective studiesurinary oxalateurinary oxalate reductionMore Related Videos
Related Concept Videos
01:24Drugs for Peptic Ulcer Disease: Sucralfate as Mucosal Protective Agents
747
In the intricate landscape of the gastric lumen, excessive acid secretion disrupts the natural defense mechanisms, weakening the mucus-bicarbonate barrier. This vulnerability allows pepsin to infiltrate epithelial cells, digesting mucosal proteins and triggering erosion, leading to ulcer formation.
In this scenario, mucosal protective agents like sucralfate play an essential role. Sucralfate, a complex of sulfated sucrose and aluminum hydroxide, demonstrates its usefulness in acidic conditions,...
In this scenario, mucosal protective agents like sucralfate play an essential role. Sucralfate, a complex of sulfated sucrose and aluminum hydroxide, demonstrates its usefulness in acidic conditions,...
747
01:16Crossover Experiments
3.8K
Crossover experiments, also called the repeated-measurements design, is a study design in which all experimental units are exposed to all treatments in different periods. Crossover experiments are generally used in psychology, the pharmaceutical industry, agriculture, and medicine.
Crossover designs are performed even with smaller sample sizes since the samples can act as their controls. These are better than simple randomized trials since patients are exposed to all the treatments.
Crossover designs are performed even with smaller sample sizes since the samples can act as their controls. These are better than simple randomized trials since patients are exposed to all the treatments.
3.8K
01:28Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies
218
Peptic ulcer disease (PUD) presents with diverse symptoms depending on the location and severity of the ulcer. Clinical manifestations of peptic ulcer include dull pain and a burning sensation in the mid-epigastric region.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
218
01:15Pyruvate Oxidation
162.5K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
162.5K
01:31Acid Suppressive Drugs for Peptic Ulcer Disease: Antacids
453
In the complex environment of the gastric lumen, excessive acid secretion can lead to the formation or worsening of ulcers within the delicate mucosal layer. Antacids, such as sodium bicarbonate and calcium carbonate, provide relief by neutralizing this acid, transforming it into harmless salt and water. This neutralization process raises the gastric pH from a highly acidic level of 1 to a more basic 3-4, reducing the acidity within the stomach.
However, this neutralization reaction between...
However, this neutralization reaction between...
453
01:28Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists
579
Histamine H2 receptors, which are intricately located on the basolateral membrane of parietal cells, play a crucial role in modulating gastric acid secretion. When released from enterochromaffin-like cells, histamine engages H2 receptors, initiating the cyclic AMP (cAMP) pathway. In this pathway, adenylyl cyclase converts ATP into cAMP, elevating intracellular cAMP levels. The activation of protein kinase A follows, stimulating the proton pump. This stimulation prompts the secretion of hydrogen...
579