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Upregulated IncRNA WTAPP1 in Triple Negative Breast Cancer Predicts Survival.

Yuguang Ma1, Liang Liang1, Yinbin Zhang1

  • 1Department of Oncology, The Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an City, Shanxi Province, 710004, P.R. China.

Critical Reviews in Eukaryotic Gene Expression
|April 4, 2022
PubMed
Summary

WTAPP1 promotes triple-negative breast cancer (TNBC) cell proliferation by downregulating miR-34a, impacting EEF2K expression. High WTAPP1 levels in TNBC tissues correlate with poor patient survival.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Endothelial progenitor cell migration and angiogenesis are vital for cancer development.
  • WTAPP1's role in these processes suggests potential involvement in cancer biology.
  • Triple-negative breast cancer (TNBC) is an aggressive subtype with limited treatment options.

Purpose of the Study:

  • To investigate the role of WTAPP1 in triple-negative breast cancer (TNBC).
  • To explore the relationship between WTAPP1, miR-34a, and EEF2K in TNBC.
  • To determine the prognostic significance of WTAPP1 expression in TNBC patients.

Main Methods:

  • Analysis of WTAPP1 and miR-34a expression in 68 TNBC patient tissues.
  • Reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot to assess gene and protein levels.
  • Cell proliferation, invasion, migration, and apoptosis assays following gene overexpression via transient transfection.

Main Results:

  • WTAPP1 was upregulated in TNBC tissues and associated with poor survival.
  • miR-34a was downregulated in TNBC and negatively correlated with WTAPP1.
  • Overexpression of WTAPP1 increased EEF2K expression, promoting TNBC cell proliferation, invasion, and migration.

Conclusions:

  • WTAPP1 promotes TNBC progression, potentially by downregulating miR-34a and upregulating its target, EEF2K.
  • WTAPP1 expression serves as a potential biomarker for poor prognosis in TNBC.
  • Targeting the WTAPP1/miR-34a/EEF2K axis may offer therapeutic strategies for TNBC.