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A ratiometric ESIPT fluorescent probe for detection of anticancer-associated H2O2 level in vitro and in vivo
Miao Chen1, Zhenhao Liang1, Xuhong Fan2
1MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China; Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Abstract:
ROS is a significant factor in the cancer treatment mechanism. The monitoring anticancer-associated H2O2 level plays a vital role in the anticancer mechanistic exploration in pathology and physiology. Herein we synthesized a ratiometric fluorescent probe (HBQ-L) to detect and image H2O2 based on excited-state intramolecular proton transfer. HBQ-L had a high sensitivity (231-fold) with a low detection limit (28.5 nM) for monitoring H2O2 in solution. HBQ-L showed good mitochondrial-targeting and successfully detected both exo-/endogenous H2O2 in A549 cells. Furthermore, HBQ-L was used to ratiometric monitor H2O2 level in anticancer reagent DOX-treated cells or zebrafish. Importantly, it was employed to access the monitoring H2O2 in the A549 tumor-bearing mice.
Insights
Researchers developed a novel fluorescent probe (HBQ-L) to detect hydrogen peroxide (H2O2), a key factor in cancer treatment. This probe enables sensitive monitoring of H2O2 levels in cells and tumor-bearing mice, aiding anticancer research.
Area of Science:
- Biomedical Engineering
- Chemical Biology
- Cancer Research
Background:
- Reactive oxygen species (ROS) play a crucial role in cancer treatment mechanisms.
- Monitoring hydrogen peroxide (H2O2) levels is vital for understanding anticancer mechanisms in pathology and physiology.
Purpose of the Study:
- To synthesize a ratiometric fluorescent probe for detecting and imaging H2O2.
- To investigate the probe's utility in monitoring H2O2 in biological systems, including cancer models.
Main Methods:
- Synthesis of a ratiometric fluorescent probe (HBQ-L) utilizing excited-state intramolecular proton transfer.
- Evaluation of probe sensitivity and detection limit for H2O2 in solution.
- Assessment of mitochondrial targeting and H2O2 detection in A549 cells, DOX-treated cells, zebrafish, and A549 tumor-bearing mice.
Main Results:
- The HBQ-L probe exhibited high sensitivity (231-fold) and a low detection limit (28.5 nM) for H2O2.
- HBQ-L demonstrated effective mitochondrial targeting and successful detection of both exogenous and endogenous H2O2 in A549 cells.
- The probe enabled ratiometric monitoring of H2O2 levels in DOX-treated cells, zebrafish, and importantly, in tumor-bearing mice.
Conclusions:
- HBQ-L is a sensitive and effective ratiometric fluorescent probe for H2O2 detection and imaging.
- The probe's mitochondrial targeting and in vivo applicability make it a valuable tool for anticancer research.
- HBQ-L facilitates real-time monitoring of H2O2 in complex biological systems, aiding mechanistic studies of cancer treatment.
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