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Updated: Sep 28, 2025

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
Published on: March 10, 2020
Circular RNA translation: novel protein isoforms and clinical significance
Shuo-Yang Wen1, Javeria Qadir1, Burton B Yang1
1Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, Toronto, ONT, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ONT, Canada.
Abstract:
In recent years, significant attention has focused on circular RNA (circRNA) translation to determine its clinical significance. Cap-independent translation of circRNAs driven by an internal ribosome entry site (IRES) or an N6-methyladenosine (m6A)-containing short sequence is different from the canonical cap-dependent translation of linear mRNAs. New proteins or isoforms possessing novel physiological roles can be generated from translatable circRNAs. The present review describes the elements involved in circRNA translation, and the functions of the translated novel protein isoforms in human diseases. Bifunctional characteristics of translatable circRNAs exerted by the circRNAs and the translated proteins are also discussed. Furthermore, various molecular strategies that could be used as appropriate therapeutic options are proposed.
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