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Aptamer-Based Cell Nucleus Imaging via Expansion Microscopy.

Yue Sun1,2, Zhicheng Zhang1,2, Tao Bing3,4

  • 1Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China.

Analytical Chemistry
|April 5, 2022
PubMed
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This summary is machine-generated.

This study introduces an aptamer-based expansion microscopy (ExM) method for high-resolution imaging. The novel technique allows clear visualization of cellular structures like chromatin morphology using conventional microscopes.

Area of Science:

  • Biotechnology
  • Microscopy
  • Molecular Biology

Background:

  • Expansion microscopy (ExM) enables nanoscale imaging with conventional microscopes.
  • Existing ExM methods may have limitations in specific molecular targeting and integration.

Purpose of the Study:

  • To develop and validate an aptamer-based ExM strategy for high-resolution cellular imaging.
  • To demonstrate the utility of aptamers in molecular recognition, fluorescence reporting, and gel anchoring within ExM.

Main Methods:

  • Chemically labeling a nucleus-targeting aptamer (Ch4-1) with a dye and acrydite.
  • Utilizing the dual-labeled aptamer (Ac-Ch4-1-FAM) for molecular recognition and gel anchoring in polyacrylamide gel.
  • Applying confocal microscopy for high-resolution imaging after gel expansion.

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Main Results:

  • The aptamer-based ExM successfully achieved nanoscale imaging of cell nuclei.
  • High-resolution imaging of chromatin morphology during different mitotic stages was accomplished.
  • Multicolor ExM imaging was demonstrated by combining aptamers with antibodies and fluorescent dyes.

Conclusions:

  • Aptamer-based ExM provides a simple and effective method for super-resolution imaging.
  • This technique offers a promising approach for visualizing cellular and tissue structures with enhanced resolution.
  • The facile aptamer labeling and simple expansion process facilitate its application.