Hydroxyapatite Fabrication for Enhancing Biohydrogen Production from Glucose Dark Fermentation

Area of Science:

• Biotechnology
• Environmental Science
• Microbiology

Background:

• Maintaining pH balance and enzyme activity is crucial for efficient biohydrogen production.
• Hydroxyapatite (HA) is known to influence chemical and biological systems.

Purpose of the Study:

• To synthesize and characterize hydroxyapatite (HA) for biohydrogen (bioH₂) production from glucose.
• To investigate the effect of HA on bioH₂ yield and microbial community structure.

Main Methods:

• Hydroxyapatite synthesized via coprecipitation.
• Biohydrogen production experiments using glucose as substrate with varying HA concentrations.
• Microbial community analysis using 16S rRNA sequencing.

Main Results:

• Optimal HA dosage of 400 mg/L resulted in a 55.80% increase in bioH₂ yield (182.33 mL/g glucose).
• HA facilitated organic substance degradation and increased soluble microbial products (SMPs).
• HA significantly increased the abundance of Firmicutes (35.27% to 76.41%) and Clostridium sensu stricto 1 (15.33% to 45.17%).

Conclusions:

• Hydroxyapatite is an effective additive for enhancing biohydrogen production from glucose.
• The mechanism involves improved enzyme activity, organic matter breakdown, and a shift in microbial community favoring hydrogen-producing bacteria.
• HA shows potential for improving bioH₂ production from organic wastewater.