Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

53.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.2K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

500
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
500
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.6K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.6K
Homologous Recombination02:31

Homologous Recombination

53.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
53.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering a Transmembrane Receptor for Coacervate-Based Artificial Cells.

Journal of the American Chemical Society·2026
Same author

PhaseXplorer Creates High-Dimensional Phase Diagrams with Closed-Loop Active Learning.

ACS nano·2025
Same author

Bayesian Optimization for Multicomponent Supramolecular Systems.

Journal of the American Chemical Society·2025
Same author

Challenges and opportunities in DNA computing and data storage.

Nature nanotechnology·2025
Same author

A microfluidic platform for extraction and analysis of bacterial genomic DNA.

Lab on a chip·2025
Same author

DNA Origami Barcodes for Immunostaining.

ACS applied materials & interfaces·2025

Related Experiment Video

Updated: Sep 28, 2025

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
06:37

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Published on: February 5, 2021

3.2K

Protocellular CRISPR/Cas-Based Diffusive Communication Using Transcriptional RNA Signaling.

Shuo Yang1, Alex Joesaar2, Bas W A Bögels1

  • 1Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MB, Eindhoven, The Netherlands.

Angewandte Chemie (International Ed. in English)
|April 6, 2022
PubMed
Summary

Synthetic protocells with CRISPR/Cas systems can process information and communicate using RNA signals. This research advances molecular information platforms and distributed cellular computing.

Keywords:
DNAEnzymesMolecular CommunicationSynthetic Protocells

More Related Videos

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.8K
CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

1.5K

Related Experiment Videos

Last Updated: Sep 28, 2025

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
06:37

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Published on: February 5, 2021

3.2K
Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.8K
CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

1.5K

Area of Science:

  • Synthetic biology
  • Biochemistry
  • Molecular engineering

Background:

  • Protocells offer a model for primitive cells and platforms for molecular information processing.
  • Enzyme-driven biomolecular circuits are key to protocell function and information exchange.
  • CRISPR/Cas systems and DNA nanotechnology provide tools for precise molecular manipulation.

Purpose of the Study:

  • To engineer protocells capable of information processing and communication.
  • To develop a molecular information platform using synthetic transcriptional circuits and CRISPR/Cas.
  • To demonstrate intercellular communication between protocells via diffusive RNA signals.

Main Methods:

  • Construction of semipermeable protein-polymer microcompartments.
  • Implementation of synthetic transcriptional circuits for RNA production.
  • Integration of CRISPR/Cas-based DNA processing within protocells.
  • Engineering RNA strands as diffusive signaling molecules.

Main Results:

  • Established transcriptional protocells activated by external DNA strands.
  • Protocells produced functional RNA aptamers and signaling RNA molecules.
  • Engineered RNA signals triggered responses in neighboring protocells, including DNA probe activation and Cas nuclease localization.
  • Demonstrated intercellular communication mediated by RNA signals.

Conclusions:

  • CRISPR/Cas machinery and DNA nanotechnology can be combined for protocellular communication.
  • Developed protocells capable of distributed molecular information processing.
  • This work represents a step towards advanced artificial cellular systems with information processing capabilities.