Fluorescence-based method for fast quantification of active aluminums in natural and treated water

Fast quantitative determination of active aluminum (Al_a) in natural and treated water is extremely desirable. The fluorescence method based on complexation by 8-hydroxyquinoline (8-HQ) is highly promising, but the measurement could be severely interfered by hardness ions and natural organic matter (NOM). This study was devoted to refining the 8-HQ complexation-fluorescence method for measurement of Al_a by eliminating the interferences. Results showed that magnesium ions at a typical concentration in natural water could have a substantial positive interference, due to the formation of Mg-8-HQ complexes which have fluorescence regions similar to Al-8-HQ. NOM, represented by fulvic acid (FA), could not interfere the aluminum measurement considerably. It was primarily because 8-HQ has much stronger complexing ability than NOM with aluminum. Theoretical calculations showed that reducing the buffering pH (from 7.5) to 6.5 or using a masking ligand such as edetate (EDTA) could effectively alleviate the interference mainly caused by magnesium. Experimental results confirmed the theoretical predictions. Refined procedures were suggested for more accurate while fast determination of Al_a in natural or treated water. The refined method has a quantification limit of ~4 μg/L, a linear range of measurement up to 700 μg/L, and a relative standard deviation of ~0.8%.