Aptamer based probes for living cell intracellular molecules detection

Area of Science:

• Biomedical Engineering
• Molecular Biology
• Nanotechnology

Background:

• Biosensors enable real-time in vivo monitoring of biomolecules, crucial for disease diagnosis and treatment.
• Detecting intracellular molecule concentration changes indicates disease progression.
• Current methods like Western blot and PCR require complex, time-consuming cell lysis.

Purpose of the Study:

• To review fluorescence detection methods using aptamers for intracellular molecule analysis.
• To summarize principles, detection limits, and performance of various aptamer-based biosensor platforms.
• To discuss challenges and future directions in intracellular molecular biosensing.

Main Methods:

• Utilizing aptamer specificity for intracellular molecule binding.
• Employing biocompatible fluorescent materials and nanomaterials.
• Developing nanostructured biosensors for cellular entry and analysis.

Main Results:

• Aptamer-based fluorescence biosensors allow in situ monitoring of intracellular molecules in living cells.
• These biosensors overcome limitations of traditional cell lysis-dependent methods.
• Various platforms demonstrate potential for sensitive and specific intracellular detection.

Conclusions:

• Aptamer-based fluorescence biosensors represent a significant advancement for intracellular molecular detection.
• They offer a sensitive, real-time, and less invasive alternative to conventional techniques.
• Further development is needed to address current challenges and optimize clinical applications.