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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Mass Spectrometers01:16

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
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Mass Spectrometry: Overview01:19

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Related Experiment Video

Updated: Sep 27, 2025

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
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Are mass spectrometry imaging-based diagnostics becoming reality?

Erin H Seeley1

  • 1Department of Chemistry, University of Texas at Austin, Austin, TX, USA.

Proteomics. Clinical Applications
|April 8, 2022
PubMed
Summary

Mass Spectrometry Imaging (MSI) offers a faster, more comprehensive diagnostic approach than traditional methods. This advanced technique analyzes hundreds of analytes from a single tissue section, improving clinical diagnostics.

Keywords:
machine learningmass spectrometry imagingtissue diagnostics

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Related Experiment Videos

Last Updated: Sep 27, 2025

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
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Area of Science:

  • Biomedical diagnostics
  • Proteomics
  • Analytical chemistry

Background:

  • Traditional immunohistochemistry is time-consuming and requires multiple tissue sections.
  • Small biopsy sizes limit the scope of traditional diagnostic tests.
  • There is a need for more efficient and comprehensive tissue analysis methods.

Purpose of the Study:

  • To highlight advances in Mass Spectrometry Imaging (MSI) for clinical diagnostics.
  • To discuss the standardization of MSI workflows and data analysis.
  • To showcase MSI as an accessible tool for clinicians.

Main Methods:

  • Mass Spectrometry Imaging (MSI) for simultaneous analyte detection.
  • Standardization of MSI workflows.
  • Development of MSI data analysis techniques.

Main Results:

  • MSI enables the detection of hundreds to thousands of analytes from a single tissue section.
  • Significant progress has been made in standardizing MSI.
  • MSI is becoming more accessible for clinical applications.

Conclusions:

  • MSI represents a significant advancement over traditional histological diagnostics.
  • Standardized MSI workflows and data analysis are crucial for clinical adoption.
  • MSI has the potential to revolutionize patient diagnosis by providing comprehensive molecular information.