Related Experiment Video
Updated: Sep 27, 2025
Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
Published on: July 17, 2014
An NBD-based fluorescent and colorimetric chemosensor for detecting S2-: Practical application to zebrafish and water
Gyeongjin Kim1, Dongkyun Gil1, Jae Jun Lee1
1Department of Fine Chem. and New and Renewable Energy Convergence, Seoul National Univ. of Sci. and Tech. (SNUT), Seoul 01811, Republic of Korea.
Abstract:
A novel 7-nitro-1,2,3-benzoxadiazole (NBD)-based chemosensor BOP ((5-bromopyridin-2-yl)(4-(7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)piperazin-1-yl)methanone) was synthesized. BOP could detect S2- through fluorescent quenching and colorimetric change. The detection limit was calculated to be 10.9 µM through fluorescence titration. The reaction mechanism of BOP towards S2- was estimated to be thiolysis of NBD amine, producing the cleavage products, NBD-S- and BP ((5-bromopyridin-2-yl)(piperazin-1-yl)methanone). The thiolysis was demonstrated by 1H NMR titrations, ESI-mass analysis and theoretical calculations. Importantly, BOP was able to successfully monitor S2- in zebrafish and water samples. Additionally, test strips coated with BOP were applied to the in-the-field measurements of S2-.
