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Related Experiment Video

Updated: Sep 27, 2025

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

105.4K

Advances in measuring DNA methylation.

Ruixia Sun1,2, Ping Zhu1,2

  • 1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences-Peking Union Medical College, Tianjin 300020, China.

Blood Science (Baltimore, Md.)
|April 11, 2022
PubMed
Summary
This summary is machine-generated.

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This review covers advancements in DNA methylation measurement techniques, including single-cell epigenome sequencing. It provides insights for selecting and optimizing DNA methylation sequencing for research.

Area of Science:

  • Epigenetics
  • Genomics
  • Molecular Biology

Background:

  • DNA methylation is a key epigenetic mechanism regulating gene expression and genome stability.
  • Accurate measurement of DNA methylation is crucial for understanding cellular processes.
  • Recent technological advancements have improved the resolution and scope of DNA methylation analysis.

Purpose of the Study:

  • To review the evolution of methods for measuring 5-methylcytosine and its oxidized derivatives.
  • To highlight recent progress in single-cell epigenome sequencing technologies.
  • To offer guidance for selecting and optimizing DNA methylation sequencing approaches.

Main Methods:

  • Review of literature on DNA methylation detection methods.
  • Analysis of next-generation sequencing applications in epigenetics.
Keywords:
5-Methylcytosine derivativesDNA methylationNext-generation sequencingSingle-cell sequencing

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Last Updated: Sep 27, 2025

DNA Methylation: Bisulphite Modification and Analysis
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Targeted DNA Methylation Analysis by Next-generation Sequencing
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  • Discussion of single-cell sequencing technologies for epigenome analysis.

    • Main Results:

    • Significant optimization of DNA methylation measurement techniques has occurred.
    • Next-generation sequencing enables genome-wide, single-base resolution of DNA methylation.
    • Single-cell epigenome sequencing offers new possibilities for detailed epigenetic studies.

    Conclusions:

    • The development of DNA methylation measurement technologies continues to advance.
    • Single-cell epigenome sequencing represents a significant step forward in epigenetic research.
    • This review provides a valuable resource for researchers in DNA methylation analysis.