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Updated: Sep 27, 2025

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Published on: July 9, 2021
Synthesis of [7-15N]-GTPs for RNA structure and dynamics by NMR spectroscopy
Kehinde M Taiwo1, Lukasz T Olenginski1, Felix Nußbaumer2
1Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, MD 20742 USA.
Abstract:
Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-15N]-guanosine-5'-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s00706-022-02892-1.
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