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Updated: Sep 27, 2025
Biological Compatibility Profile on Biomaterials for Bone Regeneration
Published on: November 16, 2018
MTA-Based Cements: Biocompatibility and Effects on the Gene Expression of Collagen Type 1 and TGF-β1
Diana María Escobar-García1, María Guadalupe Medina-Rosas2, Ana María González-Amaro2
1Basic Sciences Laboratory, Faculty of Dentistry, San Luis Potosi University, Dr. Manuel Nava Avenue # 2, Zona Universitaria, San Luis Potosí, SLP, 78290, Mexico.
Objective:
This study sought to evaluate the biocompatibility of Neomineral Trioxide Aggregate (Neo-MTA), MTA Repair High Plasticity (MTA-HP), and Mineral Trioxide Aggregate-Angelus white (MTA-Ang) in fibroblasts of human dental pulp.
Materials And Methods:
Morphology was evaluated after 24 h of incubation. LIVE/DEAD assay and cell adhesion tests were performed at 24 h of treatment. Cell proliferation assays (MTSs) and Annexin V were performed at 48 h incubation with different treatments. The expression of Col-1 and TGF-β1 was tested by endpoint PCR at 5 days of treatment.
Results:
Morphological changes were observed in all groups. Neo-MTA and MTA-Ang were associated with increased cell viability, and all materials induced apoptosis, with a higher percentage in the MTA-HP group than in the other groups. In the LIVE/DEAD assay, there was more damage to the cell membrane in the group of cells treated with MTA-HP than in the other groups.
Conclusion:
Neo-MTA and MTA-Ang presented similar biocompatibility, and both showed greater biocompatibility than MTA-HP. MTA-HP and MTA-Ang increased Col-1A gene expression, and Neo-MTA and MTA-Ang increased TGF-β1 gene expression in a similar way.
